ABSTRACT
Aim: The aim of this study was to evaluate the synergistic effect of low radiation dose with the chemotherapeutic drug in order to find possible way to lessen the harmful effects during chemo-radiotherapy. Study Design: Randomized controlled experiment. Place and Duration of Study: Experimental Animal Unit, Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo Egypt. Methodology: Estimation of antioxidant activity of low radiation dose on oxidative stress induced by cisplatin administration at a dose of 10 mg/kg b. wt. in male albino rat. Results: Results of experiment revealed that cisplatin administration caused a significant increase in serum alanine transaminase (GPT) activity (38.58±2.060) and FSH level (8.162±1.424) accompanied with a decrease in serum albumin (3.492±0.253), and Butyry Cholein Esterase (BChE) (65.35 12.61). In Liver and testis, GSH content (68.00±2.391 & 24.93±4.778) as well as cytochromes P450 levels (0.3875±0.0727 & 0.2167±0.0459) showed a significant decrease as compared to the normal control level respectively. In addition the level of Fe, Cu and Zn showed no significant changes in liver and appeared to be significantly decrease as in case of corresponding trace elements in testis organs. On the other hand, exposing to low dose of radiation (0.5 Gy) post-cisplatin treatment effectively prevented these alterations and maintained the antioxidant status. Conclusion: Data from present results revealed that low radiation dose have the existence as an antioxidant and antitumor agents which may be useful to use as a synergistic agents with the chemotherapeutic drug.
ABSTRACT
Aim: The aim of this study was to examine the effect of ethanolic extract of coriander leaves as a potent in vivo antioxidant agent in an effort of finding possible sources of antioxidants for future use in food and pharmaceutical formulations. Study Design: Randomized controlled experiment. Place and Duration of Study: Experimental Animal Unit, Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo Egypt. Methodology: Antioxidant activity of ethanol extract of coriander leaves was estimated by oxidative stress induced by radiation exposure with the dose of 4 Gy, Silymarin was used as a reference antioxidant drug in female albino rat. Results: Results of experiment revealed that radiation exposure caused a significant increase in serum caspase3 (0.870± 0.086), alanine transaminase (ALT) activity (24.43± 5.02) as well as urea (42.53± 6.11) and creatinine (0.865± 0.064) levels with an increase in liver and kidney lipid peroxidation (MDA) (307.0± 29.22 & 285.5± 48.93) respectively, while decrease in serum albumin (3.003± 0.355), protein (8.66± 0.436) as well as glutathione (GSH) contents of liver and kidney tissues (63.24± 12.19 & 17.38± 1.414) were estimated respectively. In addition serum globuline level and albumin /globuline ratio showed no significant changes. On the other hand, the administration of coriander (600 mg/kg bw) and silymarin (70 mg/kg bw) pre-treatment effectively prevented these alterations and maintained the antioxidant status. Conclusion: Data from present results revealed that Coriandum Sativum act as an antioxidant agent due to its free radical scavenging and cytoprotective activity.
ABSTRACT
Aim: Onions have potential antioxidant properties that lower the oxidative status. The Present study was aimed to investigate the antioxidant activity of onion oil in irradiated male albino rats. Study Design: Randomized controlled experiment. Place and Duration of Study: Experimental Animal Unit, Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo Egypt. Methodology: Animals were divided to the following groups each of eight rats. Control group, Onion oil group: Rats received 200 mg/kg b.wt onion oil orally for seven days. IRR group: Rats were exposed to γ- ray as a fractionated dose of 9 Gy for 7 days, Onion oil + IRR group: Rats were administered with onion oil orally along with γ- ray exposure. At the end of this study the animals were sacrificed and the effects of onion oil against hepatotesticular oxidative damages were monitored by assaying the levels of serum alanine transferase (ALT), aspartate aminotransferase (AST), alkaline phoshatase (ALP), as well as testosterone and DHEA level. In addition both liver and testis lipid peroxidation (MDA), superoxide dismutase (SOD), and catalase (CAT) were examined. Results: The results showed a significant increase in serum ACP, AST, and ALT activities (107.7%, 91.1% and 100.2% compared to control) with a decrease in testosterone (12% compared to control) and non- significant change in DHEA. The levels of SOD (59.31±10.67 & 0.21± 0.022), CAT (0.2081± 0.022 & 0.247±0.0453) and GSH (0.32±0.046 & 3.46± 0.476) were significantly decreased in irradiated group accompanied by significant increase in both liver and testis MDA (171.2±13.69 202.2±49.34) respectively. The levels of reversal effects of irradiation were shown by Onion oil + IRR group in both liver and testis. Conclusion: Data concluded that onion oil showed the reversal effects of ionizing radiation induced hepato- testicular oxidative stress.